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Anti-BNIP3L Antibody
Catalog #: 144-06283
BNIP3L Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | O60238 |
| Gene Id | 665 |
| Gene Symbols | BNIP3L |
| Protein Name / Synonyms | BNIP3L, BNIP3a, NIX, BCL2 interacting protein 3 like |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC |
| Research Area | Autophagy |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
This gene encodes a protein that belongs to the pro-apoptotic subfamily within the Bcl-2 family of proteins. The encoded protein binds to Bcl-2 and possesses the BH3 domain. The protein directly targets mitochondria and causes apoptotic changes, including loss of membrane potential and the release of cytochrome c.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IHC 1:50 - 1:200
Format
Calculated MW
19kDa/23kDa
Observed MW
30kDa, 45kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-187 of human BNIP3L (NP_004322.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using BNIP3L antibody (144-06283) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 30s.
Immunohistochemistry of paraffin-embedded human stomach using BNIP3L antibody (144-06283) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using BNIP3L antibody (144-06283) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human lung cancer using BNIP3L antibody (144-06283) at dilution of 1:100 (40x lens).
Expiration:
12 months from the date of shipment when stored properly.