Anti-BRAF Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	P15056
Gene Id	673
Gene Symbols	BRAF
Protein Name / Synonyms	BRAF
Clonality	Polyclonal
Preparation	This BRAF antibody is generated from rabbits immunized with BRAF recombinant protein.
Concentration (lot specific)	0.5 mg/ml
Recommended Applications	Western Blotting, FC
Research Area	Cardiovascular Disease, Cancer, Ras/Rap Signaling
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

BRAF, a member of the RAF subfamily of Ser/Thr protein kinases, is involved in the transduction of mitogenic signals from the cell membrane to the nucleus. It may play a role in the postsynaptic responses of hippocampal neurons. This cytoplasmic protein is expressed in brain and testis. Defects in BRAF are involved in a wide range of cancers including lung cancer and non-Hodgkin lymphoma (NHL). This protein contains 1 zinc-dependent phorbol-ester and DAG binding domain.

Specificity

Other Names

BRAF; BRAF1; RAFB1; Serine/threonine-protein kinase B-raf; Proto-oncogene B-Raf; p94; v-Raf murine sarcoma viral oncogene homolog B1

NCBI Accession #

NP_004324.2

Format

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

84437

Recommended Dilutions

WB: 1:1000; FC: 1:10-50

	BRAF Antibody (Cat# 102-17639) western blot analysis in Hela,T47D cell line lysates (35ug/lane).This demonstrates the BRAF antibody detected the BRAF protein (arrow).
	BRAF Antibody (Cat# 102-17639) flow cytometric analysis of CEM cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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