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Anti-BTD Antibody
Catalog #: 144-06284
BTD Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P43251 |
| Gene Id | 686 |
| Gene Symbols | BTD |
| Protein Name / Synonyms | BTD, biotinidase |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
The protein encoded by this gene functions to recycle protein-bound biotin by cleaving biocytin (biotin-epsilon-lysine), a normal product of carboxylase degradation, resulting in regeneration of free biotin. The encoded protein has also been shown to have biotinyl transferase activity. Mutations in this gene are associated with biotinidase deficiency. Multiple transcript variants encoding different isoforms have been described.
Dilutions
WB 1:500 - 1:2000
IHC 1:100 - 1:200
IHC 1:100 - 1:200
Format
Calculated MW
58kDa/61kDa
Observed MW
60kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 284-543 of human BTD (NP_000051.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using BTD antibody (144-06284) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat brain using BTD antibody (144-06284) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver cancer using BTD antibody (144-06284) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human prostate using BTD antibody (144-06284) at dilution of 1:100 (40x lens).
Expiration:
12 months from the date of shipment when stored properly.