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Anti-BUB3 Antibody
Catalog #: 144-06536
BUB3 Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | O43684 |
| Gene Id | 9184 |
| Gene Symbols | BUB3 |
| Protein Name / Synonyms | BUB3, BUB3L, hBUB3, mitotic checkpoint protein BUB3 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IF |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
This gene encodes a protein involved in spindle checkpoint function. The encoded protein contains four WD repeat domains and has sequence similarity with the yeast BUB3 protein. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
Dilutions
WB 1:500 - 1:2000
IF 1:50 - 1:200
IF 1:50 - 1:200
Format
Calculated MW
36kDa/37kDa
Observed MW
40kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-328 of human BUB3 (NP_004716.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using BUB3 antibody (144-06536) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 90s.
Immunofluorescence analysis of U2OS cells using BUB3 antibody (144-06536).
Expiration:
12 months from the date of shipment when stored properly.