RayBiotech

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ISO 13485:2016 cGMP

Anti-C21orf33 Antibody

Catalog #: 144-06429

C21orf33 Polyclonal Antibody

Product Description

Specifications

Size20 µL, 100 µL
Estimated Lead Time1-2 weeks
SpeciesHuman, Mouse, Rat
Host SpeciesRabbit
Accession Number
P30042
Gene Id
8209
Gene Symbols
C21ORF33
Protein Name / Synonyms
C21orf33, ES1, GATD3, GT335, HES1, KNPH, KNPI, ES1 protein homolog, mitochondrial
ClonalityPolyclonal
IsotypeIgG
Purity
Affinity purification
BuffersPBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended ApplicationsWestern Blotting, IF
Shipping TypeBlue ice
Storage-20°C

Description

Background

This gene encodes a potential mitochondrial protein that is a member of the DJ-1/PfpI gene family. This protein is overexpressed in fetal Down syndrome brain. Alternate splicing results in multiple transcript variants.

Dilutions

WB 1:500 - 1:2000
IF 1:10 - 1:100

Format

Calculated MW
24kDa/28kDa
Observed MW
25kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 42-268 of human C21orf33 (NP_004640.4).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using C21orf33 antibody (144-06429) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 60s.
Western blot - C21orf33 Polyclonal Antibody (144-06429)

Immunofluorescence analysis of U2OS cells using C21orf33 antibody (144-06429).
Immunofluorescence - C21orf33 Polyclonal Antibody (144-06429)

Expiration:
12 months from the date of shipment when stored properly.