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Anti-CAD (Center) Antibody
Catalog #: 102-10942
Rabbit Anti-Human CAD (Center) Antibody, 400 ul
Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | P27708 |
| Gene Id | 790 |
| Gene Symbols | CAD |
| Protein Name / Synonyms | CAD (Center) |
| Clonality | Polyclonal |
| Preparation | This CAD antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 780-809 amino acids from the Central region of human CAD. |
| Concentration (lot specific) | 0.290 mg/ml |
| Recommended Applications | FC, IHC-P, Western Blotting |
| Research Area | Cardiovascular Disease |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Introduction
The de novo synthesis of pyrimidine nucleotides is required for mammalian cells to proliferate. This gene encodes a trifunctional protein which is associated with the enzymatic activities of the first 3 enzymes in the 6-step pathway of pyrimidine biosynthesis: carbamoylphosphate synthetase (CPS II), aspartate transcarbamoylase, and dihydroorotase. This protein is regulated by the mitogen-activated protein kinase (MAPK) cascade, which indicates a direct link between activation of the MAPK cascade and de novo biosynthesis of pyrimidine nucleotides.
Specificity
Predicted Reactivity
Mouse
Other Names
CAD; CAD protein; Glutamine-dependent carbamoyl-phosphate synthase; Aspartate carbamoyltransferase; Dihydroorotase
NCBI Accession #
NP_004332.2
Other Accession #
B2RQC6; NP_004332.2
Format
Type
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
242984
Recommended Dilutions
WB: 1:1000; IHC: 1:10-50; FC: 1:10-50
 | CAD Antibody (Center) (Cat# 102-10942) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the CAD antibody detected the CAD protein (arrow). |
 | CAD Antibody (Center) (Cat# 102-10942)immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of CAD Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated. |
 | CAD Antibody (Center) (Cat# 102-10942) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.