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Anti-CALB2 Antibody
Catalog #: 144-09807
CALB2 Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P22676 |
| Gene Id | 794 |
| Gene Symbols | CALB2 |
| Protein Name / Synonyms | CALB2, CAB29, CAL2, CR, calretinin |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
This gene encodes an intracellular calcium-binding protein belonging to the troponin C superfamily. Members of this protein family have six EF-hand domains which bind calcium. This protein plays a role in diverse cellular functions, including message targeting and intracellular calcium buffering. It also functions as a modulator of neuronal excitability, and is a diagnostic marker for some human diseases, including Hirschsprung disease and some cancers. Alternative splicing results in multiple transcript variants.
Dilutions
WB 1:1000 - 1:4000
IHC 1:50 - 1:100
IF 1:50 - 1:200
IHC 1:50 - 1:100
IF 1:50 - 1:200
Format
Calculated MW
31kDa
Observed MW
32kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-271 of human CALB2 (NP_001731.2).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using CALB2 antibody (144-09807) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.
Western blot analysis of extracts of various cells, using CALB2 antibody (144-09807) at 1:1000 dilution ratio through one-step method.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:4000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.
Immunohistochemistry of paraffin-embedded mouse kidney using CALB2 antibody (144-09807) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse lung using CALB2 antibody (144-09807) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of rat brain using CALB2 antibody (144-09807) at dilution of 1:100. Blue: DAPI for nuclear staining.
Expiration:
12 months from the date of shipment when stored properly.