Synonyms
SLC9A1BP antibody, CHP antibody, SLC9A1 binding protein antibody, calcineurin homologous protein antibody, calcineurin B homolog antibody, calcium-binding protein CHP antibody, calcium-binding protein p22 antibody
Specificity
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
22 kDa
Immunogen
Synthetic peptide corresponding to a region within amino acids 47 and 141 of Calcium binding protein P22 (Uniprot ID# Q99653)
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, ICC/IF: 1:100-1:1000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)]. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Immunofluorescence analysis of methanol-fixed HepG2, using Calcium binding protein P22(107-10987) antibody at 1:200 dilution.

Calcium binding protein P22 antibody [N2C2], Internal detects Calcium binding protein P22 protein at cytoplasm on SW480 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded SW480 xenograft.
Calcium binding protein P22 antibody [N2C

Sample (30 ug of whole cell lysate)
A: Hep G2
12% SDS PAGE
107-10987 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.