Anti-CALD1 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	Q05682
Gene Id	800
Gene Symbols	CALD1
Protein Name / Synonyms	CALD1, CDM, H-CAD, HCAD, L-CAD, LCAD, NAG22, caldesmon
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IHC
Shipping Type	Blue ice
Storage	-20°C

Description

Background

This gene encodes a calmodulin- and actin-binding protein that plays an essential role in the regulation of smooth muscle and nonmuscle contraction. The conserved domain of this protein possesses the binding activities to Ca(2+)-calmodulin, actin, tropomyosin, myosin, and phospholipids. This protein is a potent inhibitor of the actin-tropomyosin activated myosin MgATPase, and serves as a mediating factor for Ca(2+)-dependent inhibition of smooth muscle contraction. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:200

Format

Calculated MW

61kDa/62kDa/64kDa/65kDa/93kDa

Observed MW

85kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human CALD1 (NP_004333.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using CALD1 antibody (144-05366) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.
Western blot - CALD1 antibody (144-05366)

Immunohistochemistry of paraffin-embedded human liver injury using CALD1 antibody (144-05366) at dilution of 1:100 (40x lens).
Immunohistochemistry - CALD1 antibody (144-05366)

Immunohistochemistry of paraffin-embedded human gastric cancer using CALD1 antibody (144-05366) at dilution of 1:100 (40x lens).
Immunohistochemistry - CALD1 antibody (144-05366)

Expiration:

12 months from the date of shipment when stored properly.

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