RayBiotech

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ISO 13485:2016 cGMP

Anti-CAMK2B Antibody

Catalog #: 144-02508

CAMK2B Polyclonal Antibody

Product Description

Specifications

Size20 µL, 100 µL
Estimated Lead Time1-2 weeks
SpeciesHuman, Mouse, Rat
Host SpeciesRabbit
Accession Number
Q13554
Gene Id
816
Gene Symbols
CAMK2B
Protein Name / Synonyms
CAMK2B, CAM2, CAMK2, CAMKB, calcium/calmodulin dependent protein kinase II beta
ClonalityPolyclonal
IsotypeIgG
Purity
Affinity purification
BuffersPBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended ApplicationsWestern Blotting
Shipping TypeBlue ice
Storage-20°C

Description

Background

The product of this gene belongs to the serine/threonine protein kinase family and to the Ca(2+)/calmodulin-dependent protein kinase subfamily. Calcium signaling is crucial for several aspects of plasticity at glutamatergic synapses. In mammalian cells, the enzyme is composed of four different chains: alpha, beta, gamma, and delta. The product of this gene is a beta chain. It is possible that distinct isoforms of this chain have different cellular localizations and interact differently with calmodulin. Alternative splicing results in multiple transcript variants.

Dilutions

WB 1:500 - 1:2000

Format

Calculated MW
50-60kDa/72kDa
Observed MW
51kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human CAMK2B (NP_742078.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using CAMK2B antibody (144-02508) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Western blot - CAMK2B antibody (144-02508)

Expiration:
12 months from the date of shipment when stored properly.