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Anti-Can f1 Antibody
Catalog #: 130-10028
Rabbit Anti-Can f1 Antibody
Product Description
Specifications
| Size | 20 µg, 100 µg, 200 µg, 500 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Canine (dog) |
| Accession Number | O18873 |
| Gene Id | 403830 |
| Clonality | Polyclonal |
| Immunogen | Immunogen was a partial length of recombinant protein of Canis familiaris allergen 1. This antibody was produced from a rabbit immunized with the immunogen. The IgG fraction was purified from rabbit serum by ammonium sulphate precipitation followed by Protein A/G affinity chromatography. |
| Recommended Applications | ELISA (recommended work dilution= 1:640000 (at least detecting 1.6ng/ml)) |
| Antigen Catalog Number | 230-00022 |
| Reconstitution | Supplied as lyophilized and purified antibody originally containing PBS, without Preservative Stabilizers, such as Sodium Azide. The final concentration is indicated on the shipping vial. The antibody is stable for at least years from the data of receipt when stored at -20°C to -70°C. Reconstituted antibody (recommended with H20 1 mg/ml) can also be aliquoted and stored frozen at -20°C to -70°C in a manual defrost freezer for months without detectable loss activity. Upon reconstitution, the antibody can also be stored over months at 4°C. Please avoid freeze-thaw cycles. |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Description
Description
Canis familiaris allergen 1 (Can f 1), which found in dog hair and dander extract, was a protein with molecular weight reported to range from 21 to 25 kD. It binds immunoglobulin E (IgE) from the majority of dog-allergic subjects.
Allergy to the domestic dog (Canis familiaris) is prominent worldwide. Dog allergens can be detected not only in houses where dogs are kept as pets but also in other places such as schools and day care centres where dogs are not present on a regular basis. Chronic exposure to dog and other indoor allergens, including mite and cat, may lead to allergic sensitization and contribute to the development of bronchial hyperreactivity and asthma in children.
Allergy to the domestic dog (Canis familiaris) is prominent worldwide. Dog allergens can be detected not only in houses where dogs are kept as pets but also in other places such as schools and day care centres where dogs are not present on a regular basis. Chronic exposure to dog and other indoor allergens, including mite and cat, may lead to allergic sensitization and contribute to the development of bronchial hyperreactivity and asthma in children.
Specificity
The antibody can specifically bind to its immunogen, and did not show any cross reactivity with unrelated antigens in ELISA. The specificity for binding to recombinant protein, cellular protein and native antigen is not defined. Cross reactivity with the protein from other species has not yet been tested.
Storage
Keep it at 4°C if intended for use within a month, otherwise, Store at -20°C. Minimize freezing and thawing when use.
References
- Konieczny A, Morgenstern JP, Bizinkauskas CB, Lilley CH, Brauer AW, Bond JF, Aalberse RC, Wallner BP, Kasaian MT(1997 ). The major dog allergens, Can f 1 and Can f 2, are salivary lipocalin proteins: cloning and immunological characterization of the recombinant forms. Immunology. 92(4):577-86.
Expiration:
12 months from the date of shipment when stored properly.