Anti-CAPG Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	P40121
Gene Id	822
Gene Symbols	CAPG
Protein Name / Synonyms	CAPG, AFCP, HEL-S-66, MCP, macrophage-capping protein
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IHC
Shipping Type	Blue ice
Storage	-20°C

Description

Background

This gene encodes a member of the gelsolin/villin family of actin-regulatory proteins. The encoded protein reversibly blocks the barbed ends of F-actin filaments in a Ca2+ and phosphoinositide-regulated manner, but does not sever preformed actin filaments. By capping the barbed ends of actin filaments, the encoded protein contributes to the control of actin-based motility in non-muscle cells. Alternatively spliced transcript variants have been observed for this gene.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:200

Format

Calculated MW

36kDa/38kDa

Observed MW

38kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-348 of human CAPG (NP_001738.2).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using CAPG Antibody (144-07324) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
Western blot - [KO Validated] CAPG Polyclonal Antibody (144-07324)

Western blot analysis of extracts of various cell lines, using CAPG Antibody (144-07324) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Western blot - [KO Validated] CAPG Polyclonal Antibody (144-07324)

Western blot analysis of extracts from normal (control) and CAPG knockout (KO) HeLa cells, using CAPG antibody (144-07324) at 1:1000 dilution.
Western blot - [KO Validated] CAPG Polyclonal Antibody (144-07324)

Immunohistochemistry of paraffin-embedded human lung cancer using CAPG Antibody (144-07324) at dilution of 1:100 (40x lens).
Immunohistochemistry - [KO Validated] CAPG Polyclonal Antibody (144-07324)

Immunohistochemistry of paraffin-embedded human liver cancer using CAPG Antibody (144-07324) at dilution of 1:100 (40x lens).
Immunohistochemistry - [KO Validated] CAPG Polyclonal Antibody (144-07324)

Expiration:

12 months from the date of shipment when stored properly.

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