Anti-CARD8 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	Q9Y2G2
Gene Id	22900
Gene Symbols	CARD8
Protein Name / Synonyms	CARD8, CARDINAL, DACAR, DAKAR, NDPP, NDPP1, TUCAN, caspase recruitment domain family member 8
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.09% Sodium azide, 50% glycerol, pH 7.3
Recommended Applications	Western Blotting
Research Area	Cancer, Inflammation
Shipping Type	Blue ice
Storage	-20°C

Description

Background

The protein encoded by this gene belongs to the caspase recruitment domain (CARD)-containing family of proteins, which are involved in pathways leading to activation of caspases or nuclear factor kappa-B (NFKB). This protein may be a component of the inflammasome, a protein complex that plays a role in the activation of proinflammatory caspases. It is thought that this protein acts as an adaptor molecule that negatively regulates NFKB activation, CASP1-dependent IL1B secretion, and apoptosis. Polymorphisms in this gene may be associated with a susceptibility to rheumatoid arthritis. Alternatively spliced transcript variants have been described for this gene.

Dilutions

WB 1:500 - 1:2000

Format

Calculated MW

32kDa/44kDa/48kDa/55kDa/60kDa

Observed MW

55kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human CARD8 (NP_003796.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using CARD8 antibody (144-00293) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Western blot - CARD8 Polyclonal Antibody (144-00293)

Expiration:

12 months from the date of shipment when stored properly.

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