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Anti-CASP2 Antibody
Catalog #: 144-05724
CASP2 Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P42575 |
| Gene Id | 835 |
| Gene Symbols | CASP2 |
| Protein Name / Synonyms | CASP2, CASP-2, ICH1, NEDD-2, NEDD2, PPP1R57, caspase-2 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC |
| Research Area | Apoptosis |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Caspases mediate cellular apoptosis through the proteolytic cleavage of specific protein substrates. The encoded protein may function in stress-induced cell death pathways, cell cycle maintenance, and the suppression of tumorigenesis. Increased expression of this gene may play a role in neurodegenerative disorders including Alzheimer's disease, Huntington's disease and temporal lobe epilepsy. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IHC 1:50 - 1:200
Format
Calculated MW
10kDa/34kDa/50kDa
Observed MW
60kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 153-452 of human CASP2 (NP_116764.2).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of Jurkat cells, using CASP2 antibody (144-05724) at 1:1000 dilution. Jurkat cells were treated by Etoposide (25 uM) for 5 hours
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human gastric cancer using CASP2 Antibody (144-05724) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver cancer using CASP2 Antibody (144-05724) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human breast using CASP2 Antibody (144-05724) at dilution of 1:100 (40x lens).
Expiration:
12 months from the date of shipment when stored properly.