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Anti-CASP2 (Center) Antibody
Catalog #: 102-11943
Rabbit Anti-Human CASP2 (Center) Antibody, 400 ul
Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | P42575 |
| Gene Id | 835 |
| Gene Symbols | CASP2 |
| Protein Name / Synonyms | CASP2 (Center) |
| Clonality | Polyclonal |
| Preparation | This CASP2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 198-226 amino acids from the Central region of human CASP2. |
| Concentration (lot specific) | 2.0 mg/ml |
| Recommended Applications | Western Blotting, IHC-P, FC |
| Research Area | Apoptosis |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Introduction
CASP2 is a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. The protein exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. The proteolytic cleavage of this protein is induced by a variety of apoptotic stimuli.
Specificity
Other Names
CASP2; ICH1; NEDD2; Caspase-2; Neural precursor cell expressed developmentally down-regulated protein 2; Protease ICH-1; Caspase-2 subunit p18; Caspase-2 subunit p13; Caspase-2 subunit p12
NCBI Accession #
NP_001215.1;NP_116764.2;NP_116765.2
Format
Type
Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
50685
Recommended Dilutions
WB: 1:1000; IHC: 1:10-50; FC: 1:10-50
 | Western blot analysis of CASP2 antibody (Center) (Cat# 102-11943) in HL60, K562, Ramos and NIH-3T3 cell line lysates(35ug/lane). CASP2 (arrow) was detected using the purified Pab. |
 | Formalin-fixed and paraffin-embedded human lung with CASP2 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. |
 | CASP2 Antibody (Center) (Cat# 102-11943) flow cytometric analysis of HL60 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.