Anti-CASP3 (C-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	P42574
Gene Id	836
Gene Symbols	CASP3
Protein Name / Synonyms	CASP3 (C-term)
Clonality	Polyclonal
Preparation	This CASP3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 219-248 amino acids from the C-terminal region of human CASP3.
Concentration (lot specific)	2.0 mg/ml
Recommended Applications	Western Blotting, IHC-P, FC
Research Area	Apoptosis, Cancer, Inflammation, Autophagy
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. Alternative splicing of this gene results in two transcript variants that encode the same protein. [provided by RefSeq].

Specificity

Predicted Reactivity

Mouse, Rat, Monkey, Rabbit

Other Names

CASP3; CPP32; Caspase-3; Apopain; Cysteine protease CPP32; Protein Yama; SREBP cleavage activity 1; Caspase-3 subunit p17; Caspase-3 subunit p12

NCBI Accession #

NP_004337.2;NP_116786.1

Other Accession #

P55213; Q8MJC3; P70677; Q2PFV2; NP_004337.2; NP_116786.1

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

31608

Recommended Dilutions

WB: 1:1000; IHC: 1:50-100; FC: 1:10-50

	CASP3 Antibody (C-term) (Cat# 102-11045) western blot analysis in MDA-MB435 cell line lysates (35ug/lane).This demonstrates the CASP3 antibody detected the CASP3 protein (arrow).
	CASP3 Antibody (C-term) (Cat# 102-11045)immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of CASP3 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
	CASP3 Antibody (C-term) (Cat# 102-11045) flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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