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Anti-CASP9 Antibody
Catalog #: 144-02636
CASP9 Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P55211 |
| Gene Id | 842 |
| Gene Symbols | CASP9 |
| Protein Name / Synonyms | CASP9, APAF-3, APAF3, ICE-LAP6, MCH6, PPP1R56, caspase-9 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF |
| Research Area | Apoptosis |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein can undergo autoproteolytic processing and activation by the apoptosome, a protein complex of cytochrome c and the apoptotic peptidase activating factor 1; this step is thought to be one of the earliest in the caspase activation cascade. This protein is thought to play a central role in apoptosis and to be a tumor suppressor. Alternative splicing results in multiple transcript variants.
Dilutions
WB 1:200 - 1:500
IHC 1:50 - 1:200
IF 1:50 - 1:100
IHC 1:50 - 1:200
IF 1:50 - 1:100
Format
Calculated MW
17kDa/30kDa/36kDa/46kDa
Observed MW
46kDa
Immunogen
Recombinant protein of human CASP9
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using CASP9 antibody (144-02636) at 1:300 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 60s.
Immunohistochemistry of paraffin-embedded rat lung using CASP9 Antibody (144-02636) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using CASP9 Antibody (144-02636) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of A549 cells using CASP9 antibody (144-02636).
Expiration:
12 months from the date of shipment when stored properly.