Anti-CBFA2T2 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	O43439
Gene Id	9139
Gene Symbols	CBFA2T2
Protein Name / Synonyms	CBFA2T2, EHT, MTGR1, ZMYND3, p85, protein CBFA2T2
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IF
Shipping Type	Blue ice
Storage	-20°C

Description

Background

In acute myeloid leukemia, especially in the M2 subtype, the t(8;21)(q22;q22) translocation is one of the most frequent karyotypic abnormalities. The translocation produces a chimeric gene made up of the 5'-region of the RUNX1 (AML1) gene fused to the 3'-region of the CBFA2T1 (MTG8) gene. The chimeric protein is thought to associate with the nuclear corepressor/histone deacetylase complex to block hematopoietic differentiation. The protein encoded by this gene binds to the AML1-MTG8 complex and may be important in promoting leukemogenesis. Several transcript variants are thought to exist for this gene, but the full-length natures of only three have been described.

Dilutions

WB 1:500 - 1:2000
IF 1:50 - 1:100

Format

Calculated MW

28kDa/63kDa/65kDa/67kDa

Observed MW

72kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 325-604 of human CBFA2T2 (NP_005084.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using CBFA2T2 antibody (144-07033) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Western blot - CBFA2T2 antibody (144-07033)

Immunofluorescence analysis of U2OS cells using CBFA2T2 antibody (144-07033).
Immunofluorescence - CBFA2T2 antibody (144-07033)

Expiration:

12 months from the date of shipment when stored properly.

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