RayBiotech

3607 Parkway Ln Ste 200 Norcross, Georgia 30092

www.RayBiotech.com | info@raybiotech.com

770-729-2992 | +1-888-494-8555

ISO 13485:2016 cGMP

Anti-CBLC Antibody

Catalog #: 144-07789

CBLC Polyclonal Antibody

Product Description

Specifications

Size20 µL, 100 µL
Estimated Lead Time1-2 weeks
SpeciesHuman, Mouse
Host SpeciesRabbit
Accession Number
Q9ULV8
Gene Id
23624
Gene Symbols
CBLC
Protein Name / Synonyms
CBLC, CBL-3, CBL-SL, RNF57, Cbl proto-oncogene C
ClonalityPolyclonal
IsotypeIgG
Purity
Affinity purification
BuffersPBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended ApplicationsWestern Blotting, IF
Shipping TypeBlue ice
Storage-20°C

Description

Background

This gene encodes a member of the Cbl family of E3 ubiquitin ligases. Cbl proteins play important roles in cell signaling through the ubiquitination and subsequent downregulation of tyrosine kinases. Expression of this gene may be restricted to epithelial cells, and alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.

Dilutions

WB 1:500 - 1:2000
IF 1:50 - 1:100

Format

Calculated MW
47kDa/52kDa
Observed MW
52kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 245-474 of human CBLC (NP_036248.3).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using CBLC antibody (144-07789) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
Western blot - CBLC antibody (144-07789)

Immunofluorescence analysis of A549 cells using CBLC antibody (144-07789).
Immunofluorescence - CBLC antibody (144-07789)

Expiration:
12 months from the date of shipment when stored properly.