3607 Parkway Ln Ste 200 Norcross, Georgia 30092
www.RayBiotech.com | info@raybiotech.com
770-729-2992 | +1-888-494-8555
ISO 13485:2016 cGMP
Anti-CBR3 Antibody
Catalog #: 144-07545
CBR3 Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | O75828 |
| Gene Id | 874 |
| Gene Symbols | CBR3 |
| Protein Name / Synonyms | CBR3, HEL-S-25, SDR21C2, hCBR3, carbonyl reductase 3 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
Carbonyl reductase 3 catalyzes the reduction of a large number of biologically and pharmacologically active carbonyl compounds to their corresponding alcohols. The enzyme is classified as a monomeric NADPH-dependent oxidoreductase. CBR3 contains three exons spanning 11.2 kilobases and is closely linked to another carbonyl reductase gene - CBR1.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IHC 1:50 - 1:200
IF 1:50 - 1:200
Format
Calculated MW
30kDa
Observed MW
31kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 118-277 of human CBR3 (NP_001227.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using CBR3 antibody (144-07545) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
Immunohistochemistry of paraffin-embedded rat brain using CBR3 antibody (144-07545) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse stomach using CBR3 antibody (144-07545) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of A549 cells using CBR3 antibody (144-07545). Blue: DAPI for nuclear staining.
Expiration:
12 months from the date of shipment when stored properly.