Anti-CBS Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	P35520
Gene Id	875
Gene Symbols	CBS
Protein Name / Synonyms	CBS, HIP4, cystathionine beta-synthase
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IHC, IF
Shipping Type	Blue ice
Storage	-20°C

Description

Background

The protein encoded by this gene acts as a homotetramer to catalyze the conversion of homocysteine to cystathionine, the first step in the transsulfuration pathway. The encoded protein is allosterically activated by adenosyl-methionine and uses pyridoxal phosphate as a cofactor. Defects in this gene can cause cystathionine beta-synthase deficiency (CBSD), which can lead to homocystinuria. This gene is a major contributor to cellular hydrogen sulfide production. Multiple alternatively spliced transcript variants have been found for this gene.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200

Format

Calculated MW

60kDa/61kDa

Observed MW

61kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 252-551 of human CBS (NP_000062.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using CBS antibody (144-01427) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Western blot - CBS Polyclonal Antibody (144-01427)

Immunofluorescence analysis of U2OS cells using CBS antibody (144-01427). Blue: DAPI for nuclear staining.
Immunofluorescence - CBS Polyclonal Antibody (144-01427)

Expiration:

12 months from the date of shipment when stored properly.

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