[KO Validated] CBX3 Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | Q13185 |
| Gene Id | 11335 |
| Gene Symbols | CBX3 |
| Protein Name / Synonyms | CBX3, HECH, HP1-GAMMA, HP1Hs-gamma, chromobox 3 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
At the nuclear envelope, the nuclear lamina and heterochromatin are adjacent to the inner nuclear membrane. The protein encoded by this gene binds DNA and is a component of heterochromatin. This protein also can bind lamin B receptor, an integral membrane protein found in the inner nuclear membrane. The dual binding functions of the encoded protein may explain the association of heterochromatin with the inner nuclear membrane. This protein binds histone H3 tails methylated at Lys-9 sites. This protein is also recruited to sites of ultraviolet-induced DNA damage and double-strand breaks. Two transcript variants encoding the same protein but differing in the 5' UTR, have been found for this gene.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IHC 1:50 - 1:200
IF 1:50 - 1:200
Format
Calculated MW
20kDa
Observed MW
23kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-183 of human CBX3 (NP_009207.2).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using CBX3 antibody (144-02248) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 5s.![Western blot - [KO Validated] CBX3 Polyclonal Antibody (144-02248)](https://cdn.sanity.io/images/g51potcz/production/53b171ad5f420092c7ffd1b75bf014572733c20f-511x350.jpg)
Western blot analysis of extracts of various cells, using CBX3 antibody (144-02248) at 1:1000 dilution ratio through one-step method.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 5s.![Western blot - [KO Validated] CBX3 Polyclonal Antibody (144-02248)](https://cdn.sanity.io/images/g51potcz/production/e1d206a473227deea6e4a6c126e98b082c6975e9-350x350.jpg)
Western blot analysis of extracts from normal (control) and CBX3 knockout (KO) HeLa cells, using CBX3 antibody (144-02248) at 1:500 dilution.![Western blot - [KO Validated] CBX3 Polyclonal Antibody (144-02248)](https://cdn.sanity.io/images/g51potcz/production/1921da51432996f31ce7decdc439dd9d198cc2b6-350x350.jpg)
Immunohistochemistry of paraffin-embedded human lung cancer using CBX3 antibody (144-02248) at dilution of 1:200 (40x lens).![Immunohistochemistry - [KO Validated] CBX3 Polyclonal Antibody (144-02248)](https://cdn.sanity.io/images/g51potcz/production/a4c6969da103361268687ea27d03204d572d6b57-368x550.jpg)
Immunohistochemistry of paraffin-embedded human gastric using CBX3 antibody (144-02248) at dilution of 1:200 (40x lens).![Immunohistochemistry - [KO Validated] CBX3 Polyclonal Antibody (144-02248)](https://www.raybiotech.com/files/images/Antibodies/144/144-02248/5.jpg)
Expiration:
12 months from the date of shipment when stored properly.