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Anti-CCAR1 Antibody
Catalog #: 144-60405
CCAR1 Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | Q8IX12 |
| Gene Id | 55749 |
| Gene Symbols | CCAR1 |
| Protein Name / Synonyms | CCAR1, cell division cycle and apoptosis regulator 1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IF, IP |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Dilutions
WB 1:500 - 1:2000
IF 1:50 - 1:200
IP 1:50 - 1:100
IF 1:50 - 1:200
IP 1:50 - 1:100
Format
Calculated MW
131kDa/132kDa
Observed MW
155kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human CCAR1 (NP_060707.2).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using CCAR1 antibody (144-013595) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 5s.
Immunofluorescence analysis of HeLa cells using CCAR1 antibody (144-013595). Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 200ug extracts of HeLa cells using 3ug CCAR1 antibody (144-013595). Western blot was performed from the immunoprecipitate using CCAR1 antibody (144-013595) at a dilition of 1:500.
Expiration:
12 months from the date of shipment when stored properly.