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Anti-CCNA2 Antibody
Catalog #: 144-07632
CCNA2 Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P20248 |
| Gene Id | 890 |
| Gene Symbols | CCNA2 |
| Protein Name / Synonyms | CCNA2, CCN1, CCNA, cyclin-A2 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. In contrast to cyclin A1, which is present only in germ cells, this cyclin is expressed in all tissues tested. This cyclin binds and activates CDC2 or CDK2 kinases, and thus promotes both cell cycle G1/S and G2/M transitions.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IHC 1:50 - 1:200
IF 1:50 - 1:200
Format
Calculated MW
48kDa
Observed MW
49kDa
Immunogen
A synthetic peptide of human CCNA2
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using CCNA2 antibody (144-07632) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human well-differentiated squamous skin carcinoma using CCNA2 antibody (144-07632) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using CCNA2 antibody (144-07632) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using CCNA2 antibody (144-07632) at dilution of 1:100. Blue: DAPI for nuclear staining.
Expiration:
12 months from the date of shipment when stored properly.