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Anti-CCNC Antibody
Catalog #: 144-06545
CCNC Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P24863 |
| Gene Id | 892 |
| Gene Symbols | CCNC |
| Protein Name / Synonyms | CCNC, CycC, SRB11, hSRB11, cyclin-C |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
The protein encoded by this gene is a member of the cyclin family of proteins. The encoded protein interacts with cyclin-dependent kinase 8 and induces the phophorylation of the carboxy-terminal domain of the large subunit of RNA polymerase II. The level of mRNAs for this gene peaks in the G1 phase of the cell cycle. Two transcript variants encoding different isoforms have been found for this gene.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:10 - 1:100
IHC 1:50 - 1:200
IF 1:10 - 1:100
Format
Calculated MW
22kDa/33kDa
Observed MW
26kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-283 of human CCNC (NP_005181.2).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using CCNC antibody (144-06545) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 60s.
Immunohistochemistry of paraffin-embedded rat brain using CCNC antibody (144-06545) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human lung cancer using CCNC antibody (144-06545) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human colon carcinoma using CCNC antibody (144-06545) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of MCF7 cells using CCNC antibody (144-06545). Blue: DAPI for nuclear staining.
Expiration:
12 months from the date of shipment when stored properly.