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Anti-CCND1 Antibody
Catalog #: 144-11022
CCND1 Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P24385 |
| Gene Id | 595 |
| Gene Symbols | CCND1 |
| Protein Name / Synonyms | CCND1, BCL1, D11S287E, PRAD1, U21B31, cyclin D1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF, IP |
| Research Area | Cancer |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. This protein has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. Mutations, amplification and overexpression of this gene, which alters cell cycle progression, are observed frequently in a variety of tumors and may contribute to tumorigenesis.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IP 1:50 - 1:100
IHC 1:50 - 1:200
IF 1:50 - 1:200
IP 1:50 - 1:100
Format
Calculated MW
33kDa
Observed MW
37kDa
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 200 to the C-terminus of human CCND1 (NP_444284.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using CCND1 antibody (144-11022) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat brain using CCND1 antibody (144-11022) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat islets of langerhans using CCND1 antibody (144-11022) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse liver using CCND1 antibody (144-11022) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using CCND1 antibody (144-11022) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using CCND1 antibody (144-11022) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 200ug extracts of HepG2 cells, using 3 ug CCND1 antibody (144-11022). Western blot was performed from the immunoprecipitate using CCND1 antibody (144-11022) at a dilition of 1:1000.
Expiration:
12 months from the date of shipment when stored properly.