3607 Parkway Ln Ste 200 Norcross, Georgia 30092
www.RayBiotech.com | info@raybiotech.com
770-729-2992 | +1-888-494-8555
ISO 13485:2016 cGMP
Anti-CCND3 (C-term S274) Antibody
Catalog #: 102-14758
Rabbit Anti-CCND3 (C-term S274) Antibody, 400 ul
Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Accession Number | P30281 |
| Gene Id | 896 |
| Gene Symbols | CCND3 |
| Protein Name / Synonyms | CCND3 (C-term S274) |
| Clonality | Polyclonal |
| Preparation | This CCND3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 252-281 amino acids from the C-terminal region of human CCND3. |
| Concentration (lot specific) | 1.160 mg/ml |
| Recommended Applications | Western Blotting |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Introduction
Regulatory component of the cyclin D3-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity. Component of the ternary complex, cyclin D3/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex.
Specificity
Predicted Reactivity
Bovine
Other Names
CCND3;G1/S-specific cyclin-D3
NCBI Accession #
NP_001129489.1;NP_001129597.1;NP_001129598.1;NP_001751.1
Other Accession #
P30282; Q3MHH5
Format
Type
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
32520
Recommended Dilutions
WB: 1:1000
 | CCND3 Antibody (C-term S274) (Cat# 102-14758) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the CCND3 antibody detected the CCND3 protein (arrow). |
 | CCND3 Antibody (C-term S274) (Cat# 102-14758) western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).This demonstrates the CCND3 antibody detected the CCND3 protein (arrow). |
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.