Anti-CCNG1 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	P51959
Gene Id	900
Gene Symbols	CCNG1
Protein Name / Synonyms	CCNG1, CCNG, cyclin-G1
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IHC, IF
Shipping Type	Blue ice
Storage	-20°C

Description

Background

The eukaryotic cell cycle is governed by cyclin-dependent protein kinases (CDKs) whose activities are regulated by cyclins and CDK inhibitors. The protein encoded by this gene is a member of the cyclin family and contains the cyclin box. The encoded protein lacks the protein destabilizing (PEST) sequence that is present in other family members. Transcriptional activation of this gene can be induced by tumor protein p53. Two transcript variants encoding the same protein have been identified for this gene.

Dilutions

WB 1:500 - 1:1000
IHC 1:50 - 1:100
IF 1:20 - 1:100

Format

Calculated MW

18kDa/34kDa

Observed MW

30kDa

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 200 to the C-terminus of human CCNG1 (NP_004051.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using CCNG1 antibody (144-05292) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 60s.
Western blot - CCNG1 Polyclonal Antibody (144-05292)

Immunofluorescence analysis of 293T cells using CCNG1 antibody (144-05292) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence - CCNG1 Polyclonal Antibody (144-05292)

Expiration:

12 months from the date of shipment when stored properly.

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