Anti-CCT8 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	P50990
Gene Id	10694
Gene Symbols	CCT8
Protein Name / Synonyms	CCT8, C21orf112, Cctq, D21S246, PRED71, T-complex protein 1 subunit theta
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IF
Shipping Type	Blue ice
Storage	-20°C

Description

Background

This gene encodes the theta subunit of the CCT chaperonin, which is abundant in the eukaryotic cytosol and may be involved in the transport and assembly of newly synthesized proteins. Alternative splicing results in multiple transcript variants of this gene. A pseudogene related to this gene is located on chromosome 1.

Dilutions

WB 1:1000 - 1:2000
IF 1:50 - 1:200

Format

Calculated MW

51kDa/57kDa/59kDa

Observed MW

60kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 248-497 of human CCT8 (NP_001269837.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using CCT8 antibody (144-04449) at 1:7000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 5s.
Western blot - CCT8 Polyclonal Antibody (144-04449)

Western blot analysis of extracts of various cells, using CCT8 antibody (144-04449) at 1:7000 dilution ratio through one-step method.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:4000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.
Western blot - CCT8 Polyclonal Antibody (144-04449)

Expiration:

12 months from the date of shipment when stored properly.