Anti-CD163 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	Q86VB7
Gene Id	9332
Gene Symbols	CD163
Protein Name / Synonyms	CD163, M130, MM130, SCARI1, CD163 molecule
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.09% Sodium azide, 50% glycerol, pH 7.3
Recommended Applications	Western Blotting, IHC
Research Area	Adaptive Immunity
Shipping Type	Blue ice
Storage	-20°C

Description

Background

The protein encoded by this gene is a member of the scavenger receptor cysteine-rich (SRCR) superfamily, and is exclusively expressed in monocytes and macrophages. It functions as an acute phase-regulated receptor involved in the clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages, and may thereby protect tissues from free hemoglobin-mediated oxidative damage. This protein may also function as an innate immune sensor for bacteria and inducer of local inflammation. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.

Dilutions

WB 1:500 - 1:1000

Format

Calculated MW

121kDa/124kDa/125kDa

Observed MW

180kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 750-1050 of human CD163 (NP_004235.4).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using CD163 Antibody (144-08383) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.
Western blot - CD163 Polyclonal Antibody (144-08383)

Expiration:

12 months from the date of shipment when stored properly.

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