Anti-CD177 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse
Host Species	Rabbit
Accession Number	Q8N6Q3
Gene Id	57126
Gene Symbols	CD177
Protein Name / Synonyms	CD177, HNA-2a, HNA2A, NB1, NB1 GP, PRV-1, PRV1, NB1GP, CD177 antigen
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting
Research Area	Adaptive Immunity
Shipping Type	Blue ice
Storage	-20°C

Description

Background

This gene encodes a glycosyl-phosphatidylinositol (GPI)-linked cell surface glycoprotein that plays a role in neutrophil activation. The protein can bind platelet endothelial cell adhesion molecule-1 and function in neutrophil transmigration. Mutations in this gene are associated with myeloproliferative diseases. Over-expression of this gene has been found in patients with polycythemia rubra vera. Autoantibodies against the protein may result in pulmonary transfusion reactions, and it may be involved in Wegener's granulomatosis. A related pseudogene, which is adjacent to this gene on chromosome 19, has been identified.

Dilutions

WB 1:500 - 1:2000

Format

Calculated MW

15kDa/27kDa/46kDa

Observed MW

45kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 35-285 of human CD177 (NP_065139.2).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of BT-474 cells, using CD177 antibody (144-06550) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
Western blot - CD177 antibody (144-06550)

Expiration:

12 months from the date of shipment when stored properly.

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