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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P20273 |
| Gene Id | 933 |
| Gene Symbols | CD22 |
| Protein Name / Synonyms | CD22, SIGLEC-2, SIGLEC2, CD22 molecule |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IF |
| Research Area | Adaptive Immunity |
| Shipping Type | Blue ice |
| Storage | -20°C |
Dilutions
WB 1:500 - 1:2000
IF 1:10 - 1:100
IF 1:10 - 1:100
Format
Calculated MW
75kDa/84kDa/85kDa/95kDa
Observed MW
135kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 708-847 of human CD22 (NP_001762.2).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of Raji cells, using CD22 Antibody (144-05790) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Immunofluorescence analysis of U2OS cells using CD22 antibody (144-05790). Blue: DAPI for nuclear staining.
Expiration:
12 months from the date of shipment when stored properly.