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Anti-CD22 Antibody
Catalog #: 188-10782
Mouse anti-Human/Mouse/Rat CD22 Antibody
Product Description
Specifications
| Size | 20 µg, 100 µg |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Mouse |
| Accession Number | P20273 |
| Gene Id | 933 |
| Gene Symbols | CD22,SIGLEC2 |
| Protein Name / Synonyms | B-cell receptor CD22 (B-lymphocyte cell adhesion molecule) (BL-CAM) (Sialic acid-binding Ig-like lectin 2) (Siglec-2) (T-cell surface antigen Leu-14) (CD antigen CD22) |
| Immunogen | Human Raji cells were used as the immunogen for the CD22 antibody. |
| Isotype | Kappa, IgG1 |
| Purity | Protein G affinity chromatography |
| Concentration (lot specific) | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide |
| Recommended Applications | Functional Activity (Order SAF formulation) Flow Cytometry: 0.5-1ug/million cells in 0.1ml Immunofluorescence: 0.5-1ug/ml |
| Domain / Region / Terminus | Cell surface |
| Research Area | Adaptive Immunity |
| Shipping Type | Blue ice |
| Storage | ≤-20°C |
Description
Description
Recognizes a protein of 130-140kDa, identified as CD22 (also known as BL-CAM). CD22 expression is restricted to normal and neoplastic B cells and is absent from other haemopoietic cell types. In B-cell ontogeny, CD22 is first expressed in the cytoplasm of pro-B and pre-B cells, and on the surface as B cells mature to become IgD+. It is not expressed by plasma cells, CD22 is found highly expressed in follicular mantle and marginal zone B-cells, and while germinal center B-cells are relatively weak. CD22 is a member of the immunoglobulin superfamily and serves as an adhesion receptor for sialic acid-bearing ligands expressed on erythrocytes and all leukocyte classes. It also associates with tyrosine kinases and play a role in signal transduction and B-cell activation.
Storage
Store the CD22 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
Expiration:
12 months from the date of shipment when stored properly.