Anti-CD247 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	P20963
Gene Id	919
Gene Symbols	CD247
Protein Name / Synonyms	CD247, CD3-ZETA, CD3H, CD3Q, CD3Z, IMD25, T3Z, TCRZ, CD247 molecule
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IF
Research Area	Adaptive Immunity
Shipping Type	Blue ice
Storage	-20°C

Description

Background

The protein encoded by this gene is T-cell receptor zeta, which together with T-cell receptor alpha/beta and gamma/delta heterodimers, and with CD3-gamma, -delta and -epsilon, forms the T-cell receptor-CD3 complex. The zeta chain plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. Low expression of the antigen results in impaired immune response. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

Dilutions

WB 1:500 - 1:2000
IF 1:50 - 1:200

Format

Calculated MW

18kDa

Observed MW

17kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 50-164 of human CD247 (NP_932170.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of mouse skeletal muscle, using CD247 antibody (144-02058) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Western blot - CD247 antibody (144-02058)

Immunofluorescence analysis of A549 cells using CD247 antibody (144-02058). Blue: DAPI for nuclear staining.
Immunofluorescence - CD247 antibody (144-02058)

Expiration:

12 months from the date of shipment when stored properly.

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