Anti-CD272 Antibody

Product Description

Specifications

Size	100 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Gene Id	151888
Protein Name / Synonyms	CD272
Concentration (lot specific)	1 mg/ml
Recommended Applications	IHC (Formalin-fixed paraffin-embedded sections), Western blot
Research Area	Adaptive Immunity
Shipping Type	Blue ice
Storage	-20°C

Description

Synonyms

BTLA1 antibody, CD272 antibody, FLJ16065 antibody, MGC129743 antibody, BTLA antibody, B- and T-lymphocyte-associated protein antibody, B- and T-lymphocyte attenuator antibody, B and T lymphocyte attenuator antibody, B and T lymphocyte associated antibody

Specificity

Species Reactivity

Human

Format

Product Type

Primary antibody

Formulation

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Calculated Molecular Weight

33 kDa

Immunogen

Recombinant protein fragment contain a sequence corresponding to a region within amino acids 1 and 164 of CD272

Applications

Applications Tested

IHC (Formalin-fixed paraffin-embedded sections),Western blot

Application Notes

Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)]. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

CD272 antibody detects BTLA protein by Western blot analysis.
A. 30 µg A375 whole cell lysate/extract
10 % SDS-PAGE
CD272 antibody (107-11038) dilution: 1:1000

Immunohistochemical analysis of paraffin-embedded MDAMB468 xenograft, using CD272(107-11038) antibody at 1:100 dilution.

Storage

Keep as concentrated solution. Aliquot and store at -20^°C or below. Avoid multiple freeze-thaw cycles.

Expiration:

12 months from the date of shipment when stored properly.

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