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Anti-CD3E Antibody
Catalog #: 144-01753
CD3E Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P07766 |
| Gene Id | 916 |
| Gene Symbols | CD3E |
| Protein Name / Synonyms | CD3E, IMD18, T3E, TCRE, CD3e molecule |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IP |
| Research Area | Adaptive Immunity |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
The protein encoded by this gene is the CD3-epsilon polypeptide, which together with CD3-gamma, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development. Defects in this gene cause immunodeficiency. This gene has also been linked to a susceptibility to type I diabetes in women.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IP 1:50 - 1:100
IHC 1:50 - 1:200
IP 1:50 - 1:100
Format
Calculated MW
23kDa
Observed MW
23kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 25-207 of human CD3E (NP_000724.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using CD3E Antibody (144-01753) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 5s.
Immunohistochemistry of paraffin-embedded rat spleen using CD3E antibody (144-01753) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human tonsil using CD3E antibody (144-01753) at dilution of 1:100 (40x lens).
Expiration:
12 months from the date of shipment when stored properly.