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Anti-CD3e Antibody
Catalog #: 188-10772
Mouse anti-Human/Monkey CD3e Antibody
Product Description
Specifications
| Size | 20 µg, 100 µg |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Specificity | Other species not tested. |
| Host Species | Mouse |
| Accession Number | P07766 |
| Gene Id | 916 |
| Gene Symbols | CD3E,T3E |
| Protein Name / Synonyms | T-cell surface glycoprotein CD3 epsilon chain (T-cell surface antigen T3/Leu-4 epsilon chain) (CD antigen CD3e) |
| Immunogen | Stimulated human leukocytes were used as the immunogen for the CD3e antibody. |
| Isotype | Kappa, IgG2a |
| Purity | Protein G affinity chromatography |
| Concentration (lot specific) | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide |
| Recommended Applications | Functional Activity: Induce T cell activation and proliferation (Order SAF formulation) Flow Cytometry: 0.5-1ug/million cells in 0.1ml Immunofluorescence: 1-2ug/ml |
| Domain / Region / Terminus | Cell surface and cytoplasmic |
| Research Area | Adaptive Immunity |
| Shipping Type | Blue ice |
| Storage | ≤-20°C |
Description
Description
Recognizes the epsilon-chain of CD3 (Workshop V; Code: CD03.09), which consists of five different polypeptide chains (designated as gamma, delta, epsilon, zeta, and eta) with MW ranging from 16-28kDa. The CD3 complex is closely associated at the lymphocyte cell surface with the T cell antigen receptor (TCR). Reportedly, CD3 complex is involved in signal transduction to the T cell interior following antigen recognition. The CD3 antigen is first detectable in early thymocytes and probably represents one of the earliest signs of commitment to the T cell lineage. In cortical thymocytes, CD3 is predominantly intra-cytoplasmic. However, in medullary thymocytes, it appears on the T cell surface. CD3 antigen is a highly specific marker for T cells, and is present in majority of T cell neoplasms.
Storage
Store the CD3e antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
Expiration:
12 months from the date of shipment when stored properly.