3607 Parkway Ln Ste 200 Norcross, Georgia 30092
www.RayBiotech.com | info@raybiotech.com
770-729-2992 | +1-888-494-8555
ISO 13485:2016 cGMP
Anti-CD43 Antibody, Sodium Azide Free
Catalog #: 188-10174-SAF
Mouse anti-Human CD43 Antibody [Sodium Azide Free]
Product Description
Specifications
| Size | 100 µg |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Specificity | Other species not known |
| Host Species | Mouse |
| Accession Number | P16150 |
| Gene Id | 6693 |
| Gene Symbols | SPN,CD43 |
| Protein Name / Synonyms | Leukosialin (Galactoglycoprotein) (GALGP) (Leukocyte sialoglycoprotein) (Sialophorin) (CD antigen CD43) |
| Immunogen | Myeloblastic KG1 cells were used as the immunogen. |
| Isotype | Kappa, IgG1 |
| Purity | Protein G affinity chromatography |
| Concentration (lot specific) | 1 mg/ml in 1X PBS (pH 7.4), BSA free, sodium azide free |
| Recommended Applications | Western Blot: 0.5-1ug/ml FACS:0.5-1ug/million cells IF: 1-2ug/ml IHC (FFPE): 0.5-1ug/ml for 30 minutes at RT (1) Prediluted format: incubate for 30 min at RT (2) |
| Domain / Region / Terminus | Cell surface |
| Research Area | Cancer, Adaptive Immunity |
| Shipping Type | Blue ice |
| Storage | ≤-20°C |
Description
Description
This antibody recognizes a cell surface glycoprotein of 95/115/135kDa (depending upon the extent of glycosylation), identified as CD43 [Workshop IV]. 70-90% of T-cell lymphomas and 22-37% of B-cell lymphomas express CD43. No reactivity has been observed with reactive B-cells. So a B-lineage population that co-expresses CD43 is highly likely to be a malignant lymphoma, especially a low-grade lymphoma, rather than a reactive B-cell population. When CD43 antibody is used in combination with CD20 antibody, effective immunophenotyping of the lymphomas in formalin-fixed tissues can be obtained. Co-staining of a lymphoid infiltrate with CD20 and CD42 antibody argues against a reactive process and favors a diagnosis of lymphoma.
Storage
Store the CD43 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
Expiration:
12 months from the date of shipment when stored properly.