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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Accession Number | P16070 |
| Gene Id | 960 |
| Gene Symbols | CD44 |
| Protein Name / Synonyms | CD44, CDW44, CSPG8, ECMR-III, HCELL, HUTCH-I, IN, LHR, MC56, MDU2, MDU3, MIC4, Pgp1, CD44 antigen |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF |
| Research Area | Inflammation, Adaptive Immunity |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
The protein encoded by this gene is a cell-surface glycoprotein involved in cell-cell interactions, cell adhesion and migration. It is a receptor for hyaluronic acid (HA) and can also interact with other ligands, such as osteopontin, collagens, and matrix metalloproteinases (MMPs). This protein participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing, hematopoiesis, and tumor metastasis. Transcripts for this gene undergo complex alternative splicing that results in many functionally distinct isoforms, however, the full length nature of some of these variants has not been determined. Alternative splicing is the basis for the structural and functional diversity of this protein, and may be related to tumor metastasis.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:100
IHC 1:50 - 1:200
IF 1:50 - 1:100
Format
Calculated MW
3kDa/15kDa/32-53kDa/73-81kDa
Observed MW
82kDa
Immunogen
Recombinant protein of human CD44
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using CD44 antibody (144-00340) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human trachea using CD44 antibody (144-00340) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human lung cancer using CD44 antibody (144-00340) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat lung using CD44 antibody (144-00340) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat spleen using CD44 antibody (144-00340) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver using CD44 antibody (144-00340) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse spleen using CD44 antibody (144-00340) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of A431 cells using CD44 antibody (144-00340) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Expiration:
12 months from the date of shipment when stored properly.