Anti-CD47 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	Q08722
Gene Id	961
Gene Symbols	CD47
Protein Name / Synonyms	CD47, IAP, MER6, OA3, CD47 molecule
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IHC, IF
Research Area	Angiogenesis, Cancer, Inflammation, Adaptive Immunity
Shipping Type	Blue ice
Storage	-20°C

Description

Background

This gene encodes a membrane protein, which is involved in the increase in intracellular calcium concentration that occurs upon cell adhesion to extracellular matrix. The encoded protein is also a receptor for the C-terminal cell binding domain of thrombospondin, and it may play a role in membrane transport and signal transduction. This gene has broad tissue distribution, and is reduced in expression on Rh erythrocytes. Alternatively spliced transcript variants have been found for this gene.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200

Format

Calculated MW

31kDa/33kDa/35kDa

Observed MW

47kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 20-140 of human CD47 (NP_942088.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using CD47 Antibody (144-01838) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Western blot - CD47 Polyclonal Antibody (144-01838)

Immunofluorescence analysis of Jurkat cells using CD47 antibody (144-01838) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence - CD47 Polyclonal Antibody (144-01838)

Expiration:

12 months from the date of shipment when stored properly.

Featured Products