Anti-CD55 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	P08174
Gene Id	1604
Gene Symbols	CD55
Protein Name / Synonyms	CD55, CR, CROM, DAF, TC, CD55 molecule (Cromer blood group)
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IF
Research Area	Inflammation, Adaptive Immunity
Shipping Type	Blue ice
Storage	-20°C

Description

Background

This gene encodes a glycoprotein involved in the regulation of the complement cascade. Binding of the encoded protein to complement proteins accelerates their decay, thereby disrupting the cascade and preventing damage to host cells. Antigens present on this protein constitute the Cromer blood group system (CROM). Alternative splicing results in multiple transcript variants. The predominant transcript variant encodes a membrane-bound protein, but alternatively spliced transcripts may produce soluble proteins.

Dilutions

WB 1:500 - 1:2000
IF 1:50 - 1:200

Format

Calculated MW

39kDa/41kDa/48kDa/56kDa/59kDa

Observed MW

50kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 40-300 of human CD55 (NP_000565.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using CD55 antibody (144-01228) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Western blot - CD55 antibody (144-01228)

Immunofluorescence analysis of A549 cells using CD55 antibody (144-01228). Blue: DAPI for nuclear staining.
Immunofluorescence - CD55 antibody (144-01228)

Expiration:

12 months from the date of shipment when stored properly.

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