RayBiotech

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Anti-CD58 Antibody

Catalog #: 144-05684

CD58 Polyclonal Antibody

Product Description

Specifications

Size20 µL, 100 µL
Estimated Lead Time1-2 weeks
SpeciesHuman
Host SpeciesRabbit
Accession Number
P19256
Gene Id
965
Gene Symbols
CD58
Protein Name / Synonyms
CD58, LFA-3, LFA3, ag3, CD58 molecule
ClonalityPolyclonal
IsotypeIgG
Purity
Affinity purification
BuffersPBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended ApplicationsWestern Blotting
Research AreaCancer, Adaptive Immunity
Shipping TypeBlue ice
Storage-20°C

Description

Background

This gene encodes a member of the immunoglobulin superfamily. The encoded protein is a ligand of the T lymphocyte CD2 protein, and functions in adhesion and activation of T lymphocytes. The protein is localized to the plasma membrane. Alternatively spliced transcript variants have been described.

Dilutions

WB 1:500 - 1:2000

Format

Calculated MW
26kDa/27kDa/28kDa
Observed MW
70kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 40-220 of human CD58 (NP_001770.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using CD58 Antibody (144-05684) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Western blot - CD58 Antibody (144-05684)

Expiration:
12 months from the date of shipment when stored properly.