Anti-CD70 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	P32970
Gene Id	970
Gene Symbols	CD70
Protein Name / Synonyms	CD70, CD27-L, CD27L, CD27LG, TNFSF7, TNLG8A, CD70 antigen
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IF
Research Area	Cancer, Adaptive Immunity
Shipping Type	Blue ice
Storage	-20°C

Description

Background

The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for TNFRSF27/CD27. It is a surface antigen on activated, but not on resting, T and B lymphocytes. It induces proliferation of costimulated T cells, enhances the generation of cytolytic T cells, and contributes to T cell activation. This cytokine is also reported to play a role in regulating B-cell activation, cytotoxic function of natural killer cells, and immunoglobulin sythesis.

Dilutions

WB 1:500 - 1:2000
IF 1:50 - 1:200

Format

Calculated MW

21kDa/23kDa

Observed MW

23kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 39-193 of human CD70 (NP_001243.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using CD70 antibody (144-02032) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Western blot - CD70 antibody (144-02032)

Immunofluorescence analysis of U2OS cells using CD70 antibody (144-02032). Blue: DAPI for nuclear staining.
Immunofluorescence - CD70 antibody (144-02032)

Expiration:

12 months from the date of shipment when stored properly.

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