Anti-CD74 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Rat
Host Species	Rabbit
Accession Number	P04233
Gene Id	972
Gene Symbols	CD74
Protein Name / Synonyms	CD74, DHLAG, HLADG, II, Ia-GAMMA, CD74 molecule
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IHC
Research Area	Cancer, Adaptive Immunity
Shipping Type	Blue ice
Storage	-20°C

Description

Background

The protein encoded by this gene associates with class II major histocompatibility complex (MHC) and is an important chaperone that regulates antigen presentation for immune response. It also serves as cell surface receptor for the cytokine macrophage migration inhibitory factor (MIF) which, when bound to the encoded protein, initiates survival pathways and cell proliferation. This protein also interacts with amyloid precursor protein (APP) and suppresses the production of amyloid beta (Abeta). Multiple alternatively spliced transcript variants encoding different isoforms have been identified.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:200

Format

Calculated MW

18kDa/26kDa/33kDa

Observed MW

29kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 73-232 of human CD74 (NP_004346.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using CD74 antibody (144-05667) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 60s.
Western blot - CD74 antibody (144-05667)

Immunohistochemistry of paraffin-embedded human B cell lymphoma using CD74 Antibody (144-05667) at dilution of 1:100 (40x lens).
Immunohistochemistry - CD74 Antibody (144-05667)

Immunohistochemistry of paraffin-embedded human amygdalitis using CD74 Antibody (144-05667) at dilution of 1:100 (40x lens).
Immunohistochemistry - CD74 Antibody (144-05667)