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ISO 13485:2016 cGMP

Anti-CD84 Antibody

Catalog #: 144-06433

CD84 Polyclonal Antibody

Product Description

Specifications

Size20 µL, 100 µL
Estimated Lead Time1-2 weeks
SpeciesHuman
Host SpeciesRabbit
Accession Number
Q9UIB8
Gene Id
8832
Gene Symbols
CD84
Protein Name / Synonyms
CD84, LY9B, SLAMF5, hCD84, mCD84, CD84 molecule
ClonalityPolyclonal
IsotypeIgG
Purity
Affinity purification
BuffersPBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended ApplicationsWestern Blotting, IF
Research AreaAdaptive Immunity
Shipping TypeBlue ice
Storage-20°C

Description

Background

This gene encodes a membrane glycoprotein that is a member of the signaling lymphocyte activation molecule (SLAM) family. This family forms a subset of the larger CD2 cell-surface receptor Ig superfamily. The encoded protein is a homophilic adhesion molecule that is expressed in numerous immune cells types and is involved in regulating receptor-mediated signaling in those cells. Alternate splicing results in multiple transcript variants.

Dilutions

WB 1:500 - 1:2000
IF 1:50 - 1:100

Format

Calculated MW
24kDa/26kDa/30kDa/31kDa/36kDa/38kDa
Observed MW
65kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 22-225 of human CD84 (NP_003865.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using CD84 antibody (144-06433) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.
Western blot - CD84 antibody (144-06433)

Immunofluorescence analysis of HeLa cells using CD84 antibody (144-06433). Blue: DAPI for nuclear staining.
Immunofluorescence - CD84 antibody (144-06433)

Expiration:
12 months from the date of shipment when stored properly.