CDA Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Accession Number | P32320 |
| Gene Id | 978 |
| Gene Symbols | CDA |
| Protein Name / Synonyms | CDA, CDD, cytidine deaminase |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
This gene encodes an enzyme involved in pyrimidine salvaging. The encoded protein forms a homotetramer that catalyzes the irreversible hydrolytic deamination of cytidine and deoxycytidine to uridine and deoxyuridine, respectively. It is one of several deaminases responsible for maintaining the cellular pyrimidine pool. Mutations in this gene are associated with decreased sensitivity to the cytosine nucleoside analogue cytosine arabinoside used in the treatment of certain childhood leukemias.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:100
IHC 1:50 - 1:200
IF 1:50 - 1:100
Format
Calculated MW
16kDa
Observed MW
16kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-146 of human CDA (NP_001776.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using CDA antibody (144-013959) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded human liver cancer using CDA Antibody (144-013959) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human colon carcinoma using CDA Antibody (144-013959) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human esophagus using CDA Antibody (144-013959) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse lung using CDA Antibody (144-013959) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using CDA Antibody (144-013959) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse stomach using CDA Antibody (144-013959) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using CDA antibody (144-013959). Blue: DAPI for nuclear staining.
Expiration:
12 months from the date of shipment when stored properly.