Anti-CDCA3 (N-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse
Host Species	Rabbit
Accession Number	Q99618
Gene Id	83461
Gene Symbols	CDCA3
Protein Name / Synonyms	CDCA3 (N-term)
Clonality	Polyclonal
Preparation	This CDCA3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 41-69 amino acids from the N-terminal region of human CDCA3.
Concentration (lot specific)	2.0 mg/ml
Recommended Applications	Western Blotting, IHC-P, FC
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

CDCA3 is a F-box-like protein which is required for entry into mitosis. It acts by participating to E3 ligase complexes that mediate the ubiquitination and degradation of WEE1 kinase at G2/M phase.

Specificity

Other Names

CDCA3; C8; GRCC8; TOME1; Cell division cycle-associated protein 3; Gene-rich cluster protein C8; Trigger of mitotic entry protein 1

NCBI Accession #

NP_112589.1

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

28998

Recommended Dilutions

WB: 1:1000; IHC: 1:50-100; FC: 1:10-50

	Western blot analysis of CDCA3 antibody (N-term) (Cat# 102-16849) in mouse spleen tissue lysates(35ug/lane). CDCA3 (arrow) was detected using the purified Pab.
	Formalin-fixed and paraffin-embedded human brain tissue reacted with CDCA3 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
	CDCA3 Antibody (N-term) (Cat# 102-16849) flow cytometric analysis of Jurkat cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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