3607 Parkway Ln Ste 200 Norcross, Georgia 30092
www.RayBiotech.com | info@raybiotech.com
770-729-2992 | +1-888-494-8555
ISO 13485:2016 cGMP
Anti-CDH16 Antibody
Catalog #: 144-07882
CDH16 Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | O75309 |
| Gene Id | 1014 |
| Gene Symbols | CDH16 |
| Protein Name / Synonyms | CDH16, cadherin-16 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
This gene is a member of the cadherin superfamily, genes encoding calcium-dependent, membrane-associated glycoproteins. Mapped to a previously identified cluster of cadherin genes on chromosome 16q22.1, the gene localizes with superfamily members CDH1, CDH3, CDH5, CDH8 and CDH11. The protein consists of an extracellular domain containing 6 cadherin domains, a transmembrane region and a truncated cytoplasmic domain but lacks the prosequence and tripeptide HAV adhesion recognition sequence typical of most classical cadherins. Expression is exclusively in kidney, where the protein functions as the principal mediator of homotypic cellular recognition, playing a role in the morphogenic direction of tissue development. Alternatively spliced transcript variants encoding distinct isoforms have been identified.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IHC 1:50 - 1:200
Format
Calculated MW
79kDa/85kDa/87kDa/89kDa
Observed MW
110kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 19-230 of human CDH16 (NP_004053.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using CDH16 antibody (144-07882) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat brain using CDH16 antibody (144-07882) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat kidney using CDH16 antibody (144-07882) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat heart using CDH16 antibody (144-07882) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using CDH16 antibody (144-07882) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using CDH16 antibody (144-07882) at dilution of 1:100 (40x lens).
Expiration:
12 months from the date of shipment when stored properly.