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Anti-CDH2 Antibody

Catalog #: 144-03045

CDH2 Polyclonal Antibody

Product Description

Specifications

Size20 µL, 100 µL
Estimated Lead Time1-2 weeks
SpeciesHuman, Mouse, Rat
Host SpeciesRabbit
Accession Number
P19022
Gene Id
1000
Gene Symbols
CDH2
Protein Name / Synonyms
CDH2, CD325, CDHN, CDw325, NCAD, cadherin-2
ClonalityPolyclonal
IsotypeIgG
Purity
Affinity purification
BuffersPBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended ApplicationsWestern Blotting, IP
Research AreaCardiovascular Disease
Shipping TypeBlue ice
Storage-20°C

Description

Background

This gene encodes a classical cadherin and member of the cadherin superfamily. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein is proteolytically processed to generate a calcium-dependent cell adhesion molecule and glycoprotein. This protein plays a role in the establishment of left-right asymmetry, development of the nervous system and the formation of cartilage and bone.

Dilutions

WB 1:500 - 1:1000
IP 1:50 - 1:100

Format

Calculated MW
97kDa/99kDa
Observed MW
135kDa

Immunogen

A synthetic peptide of human CDH2

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using CDH2 antibody (144-03045) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
Western blot - CDH2 Polyclonal Antibody (144-03045)

Western blot analysis of extracts from normal (control) and CDH2 knockout (KO) HeLa cells, using CDH2 antibody (144-03045) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Western blot - CDH2 Polyclonal Antibody (144-03045)

Immunoprecipitation analysis of 200ug extracts of HeLa cells, using 3 ug CDH2 antibody (144-03045). Western blot was performed from the immunoprecipitate using CDH2 antibody (144-03045) at a dilition of 1:1000.
Immunoprecipitation - CDH2 Polyclonal Antibody (144-03045)

Expiration:
12 months from the date of shipment when stored properly.