Anti-CDH5 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse
Host Species	Rabbit
Accession Number	P33151
Gene Id	1003
Gene Symbols	CDH5
Protein Name / Synonyms	CDH5, 7B4, CD144, cadherin-5
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting
Research Area	Angiogenesis, Inflammation
Shipping Type	Blue ice
Storage	-20°C

Description

Background

This gene encodes a classical cadherin of the cadherin superfamily. The encoded preproprotein is proteolytically processed to generate the mature glycoprotein. This calcium-dependent cell-cell adhesion molecule is comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Functioning as a classical cadherin by imparting to cells the ability to adhere in a homophilic manner, this protein plays a role in endothelial adherens junction assembly and maintenance. This gene is located in a gene cluster in a region on the long arm of chromosome 16 that is involved in loss of heterozygosity events in breast and prostate cancer.

Dilutions

WB 1:500 - 1:1000

Format

Calculated MW

74kDa/87kDa

Observed MW

120kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 259-358 of human CDH5 (NP_001786.2).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using CDH5 antibody (144-00734) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Western blot - CDH5 Polyclonal Antibody (144-00734)

Expiration:

12 months from the date of shipment when stored properly.

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